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Cat Number:ABT2011-2
Size:200uL
Price:£229.00
Quantity:
Shipping:£12.00

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DDDDK Tag, Rabbit Polyclonal Antibody

Partner: Abbkine Scientific

Applications:WB, IF, IP
Dilutions:Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Isotype:Rabbit IgG
Immunogen:Synthetic Peptide
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Description:Featured Anti-DDDDK Tag Rabbit Polyclonal Antibody, specially designed for your taged protein quantification and localization.
Further Information:The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Shipping Conditions:Ship on cold packs
Storage:Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:Research use only

 

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A02011-wb.jpg&&Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag rabbit polyclonal antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.

A02011-wb.jpg&&Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag rabbit polyclonal antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.