Ordering Details
Cat Number:	ABT2010-2
Size:	200uL
Concentration:	1 mg/ml
Price:	£109.00
Quantity:
Shipping:	£12.00

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MSDS

DDDDK Tag [1B10] HRP, Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:	WB, IF, IP
Dilutions:	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).
Isotype:	Mouse IgG
Immunogen:	Synthetic Peptide
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:	Featured Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), specially designed for your taged protein quantification and localization.
Further Information:	The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Shipping Conditions:	Ship on cold packs
Storage:	Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:	Research use only

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A02010-if.jpg&&Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.|||A02010-wb.jpg&&Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).