Applications: | ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system. |