| Applications: | ELISA (recommended work dilution= 1: 40,000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa). |
