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130-10179B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:40,000), Western Blotting (recommended work dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10305B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5C6-E11-C2-G11-D2) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10306B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (4D2-E1-D5-G8-G11-E11) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10307B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5B9-G2-F12) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-08-0003B-50
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RayBiotech, Inc.
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Visfatin [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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RB-08-0013B-50
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RayBiotech, Inc.
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POMC [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
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230-10253-100
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RayBiotech, Inc.
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Recombinant human PLXDC1, transfected HEK293 cell lysate
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