Applications: | Dot Blot, ELISA, WB |
Species Reactivity: | Human, Chimpanzee |
Isotype: | Chicken IgY |
Immunogen: | This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. |
Purification: | L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross-reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences. |
MW: | 23354 MW |
Description: | Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot Blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee. |
Synonym: | chicken anti-L1/ORF2, chicken anti-L1-ORF2, L1 (LINE-1), Long INterspersed Element, retrotransposon ORF2 |
Shipping Conditions: | Ship on cold packs |
Storage: | Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Usage: | Research use only |