RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-IP-10-101B-50
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RayBiotech, Inc.
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Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody
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HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10010B-50
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RayBiotech, Inc.
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PAI-1 [+Biotin], Rabbit Polyclonal Antibody
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Rabbit Anti-Human PAI-1 antibody detects a target derived from a human recombinant PAI-1 protein aswell as selected cell lysates. Cross reactivity with mouse and rat PAI-1 was not determined.
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ELISA (recommended work dilution= 1: 20,000)
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BNCB0669-100
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Biotium
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p27/KIP1 [SX53G8] Biotin, Mouse Monoclonal Antibody
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IHC(p), FC, IF, IP, WB
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BNCB0669-500
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Biotium
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p27/KIP1 [SX53G8] Biotin, Mouse Monoclonal Antibody
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IHC(p), FC, IF, IP, WB
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BNCB0811-100
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BNCB0811-500
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BA1006-0.5ml
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Boster Biological Technology
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Rabbit anti-Goat IgG [H+L], Biotin
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Goat
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ELISA,IHC
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BA1006-1ml
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Boster Biological Technology
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Rabbit anti-Goat IgG [H+L], Biotin
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Goat
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ELISA,IHC
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