Western Blot analysis: The recombinant Synaptobrevin 1, 2 (each 20ng) and the extracts of Rat brain (20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human Synaptobrevin 2 (1/2,000). Proteins were visualized using a goat anti-Mouse secondary antibody conj ugated to HRP and an ECL detection system. Lane 1: Synaptobrevin 1 Lane 2: Synaptobrevin 2 Lane 3. Rat brain