Western blot of rabbit anti-Hexokinase antibody peroxidase conjugated. Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a, blocking buffer for 30 min at RT. Predicted/Observed size: 54 kDa, 54 kDa for Hexokinase.