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Cat Number:sc-413409-ACT-2

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apoL-IV CRISPR Activation Plasmid [Human 2]

Partner: Santa Cruz Biotechnology

Species Reactivity:Human
Description:20ug of transfection-ready purified plasmid DNA, suitable for up to 20 transfections. CRISPR Activation Plasmid is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression.
Further Information:CRISPR Activation Plasmid consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene. The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
Shipping Conditions:Ship at ambient
Storage:Store at -20 C
Usage:Research use only


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