MUDENG HDR Plasmid [Human]
Partner: Santa Cruz Biotechnology
| Species Reactivity: | Human |
| Description: | 20ug of transfection-ready purified plasmid DNA, suitable for up to 20 transfections. HDR Plasmids are recommended for co-transfection with corresponding CRISPR/Cas9 KO Plasmid. HDR Plasmids consists of a pool of 2-3 plasmids, each containing a homology-directed DNA repair (HDR) template corresponding to the cut sites generated by the CRISPR/Cas9 KO Plasmid. |
| Further Information: | Each HDR plasmid contains two 800 bp homology arms designed to specifically bind to the genomic DNA surrounding the corresponding Cas9-induced double strand DNA break site. Each HDR Plasmid inserts a puromycin resistance gene to enable selection of stable knockout (KO) cells and an RFP (Red Fluorescent Protein) gene to visually verify transfection. The puromycin resistance and RFP genes are flanked by two LoxP sites to allow for further processing by the Cre Vector sc-418923 |
| Shipping Conditions: | Ship at ambient |
| Storage: | Store at -20 C |
| Usage: | Research use only |
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