HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBE2D4 (Cat# RC202473, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D4 (Cat# TA810786)(1:2000). Positive lysates [LY414273] (100 ug) and [LC414273] (20 ug) can be purchased separately from OriGene.