HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DNAJB6 (Cat# RC201620, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB6 (1:2000) (Cat# TA810677). Positive lysates [LY417262] (100 ug) and [LC417262] (20 ug) can be purchased separately from OriGene.