HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1 (RC206885, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCD1. Positive lysates LY424967 (100 ug) and LC424967 (20 ug) can be purchased separately from OriGene.