HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMP4 (RC204473, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMP4. Positive lysates LY429051 (100 ug) and LC429051 (20 ug) can be purchased separately from OriGene.