Ordering Details
Cat Number:	P-2023-100
Size:	each
Price:	£170.00
Quantity:
Shipping:	£12.00

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Data Sheet

ChromaFlash Chromatin Isolation & Shearing Kit [100 preparations]

Partner: Epigentek Group Inc

Applications:	Sample Isolation
Description:	The ChromaFlash Chromatin Isolation & Shearing Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format, followed by a shearing step by sonication. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek�s one-hour ChIP method using the ChromaFlash One-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Shipping Conditions:	Ship on cold packs
Usage:	Research Use Only

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Schematic procedure of the ChromaFlash™ Chromatin Isolation & Shearing Kit.

Chromatin was extracted from six MDA-231 cell samples. 40 ul (8 ug) of chromatin were sheared in a 0.2 ml tube using the EpiSonic 1100 for 20 cycles (15 sec On and 30 sec Off). DNA was purified and then run on a gel.

The sheared chromatin was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH and MLH1 promoters by using the ChromaFlash™ One-Step ChIP Kit (P-2025) and the Methylamp™ MS-qPCR Fast Kit (P-1028).

The sheared chromatin was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH and MLH1 promoters by using the ChromaFlash™ One-Step ChIP Kit (P-2025) and the Methylamp™ MS-qPCR Fast Kit (P-1028).