Schematic procedure of chromation isolation with the EpiQuik Total Histone Extraction Kit.
Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3K9 Quantification Kit (Colorimetric) (Cat. No. P-4010).
SDS-PAGE analysis of histone extracts was prepared with the EpiQuik Total Histone Extraction Kit. 10 ug of each sample were loaded per lane (1-8).