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Cat Number:OP-0006-100
Size:each
Price:£135.00
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Shipping:£12.00

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EpiQuik Total Histone Extraction Kit [100 extractions]

Partner: Epigentek Group Inc

Applications:Sample Isolation
Description:The EpiQuik Total Histone Extraction Kit is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits or in a variety of downstream applications for histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination, and ADP-ribosylation studies.
Shipping Conditions:Ship at ambient
Usage:Research Use Only

 

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Schematic procedure of chromation isolation with the EpiQuik Total Histone Extraction Kit.

Schematic procedure of chromation isolation with the EpiQuik Total Histone Extraction Kit.

Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3K9 Quantification Kit (Colorimetric) (Cat. No. P-4010).

Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3K9 Quantification Kit (Colorimetric) (Cat. No. P-4010).

SDS-PAGE analysis of histone extracts was prepared with the EpiQuik Total Histone Extraction Kit. 10 ug of each sample were loaded per lane (1-8).

SDS-PAGE analysis of histone extracts was prepared with the EpiQuik Total Histone Extraction Kit. 10 ug of each sample were loaded per lane (1-8).