Figure 1. Comparison of commercially Hot-Start DNA Polymerase. 406 bp, 653 bp, and 320 bp fragments were amplified from 3.6 ng of human genomic DNA in 20 uL reactions using (A) NanoTaq Hot-Start DNA Polymerase, (B) DreamTaq Hot Start DNA Polymerase, (C) KAPA2G Fast HotStart PCR Kit. The programs were according to different Hot-Start DNA Polymerase suggested. The size marker is Bio-Helix 1Kb DNA Ladder RTU.
Figure 2. Efficient amplification of DNA sequences with a range of GC content. A series of DNA fragments of increasing GC content were amplified from human gDNA. NanoTaq Hot-Start DNA Polymerase was used for targets with >50% GC.
