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Cat Number:DF7156-100ul
Size:100uL
Concentration:1mg/mL
Price:£215.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

14-3-3 gamma, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:WB, IHC, IF/ICC, ELISA
Species Reactivity:Human, Mouse, Rat
Isotype:Rabbit IgG
Immunogen:A synthesized peptide derived from human 14-3-3 gamma, corresponding to a region within the internal amino acids.
Purification:The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:Unconjugated
MW:28kDa
Shipping Conditions:Ship on cold packs
Storage:Store at -20 C. Stable for 12 months from date of receipt.
Usage:Research use only

 

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Western blot analysis of extracts of Jurkat,, using YWHAG antibody. The lane on the left is treated with the antigen-specific peptide.

Western blot analysis of extracts of Jurkat,, using YWHAG antibody. The lane on the left is treated with the antigen-specific peptide.

DF7156 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

DF7156 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

DF7156 at 1/100 staining mouse brain tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

DF7156 at 1/100 staining mouse brain tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.