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Cat Number:DF3493-200ul
Size:200uL
Concentration:1mg/mL
Price:£269.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

14-3-3 gamma, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:WB, IHC, IF/ICC, ELISA
Species Reactivity:Human, Mouse, Rat
Isotype:Rabbit IgG
Immunogen:A synthesized peptide derived from human 14-3-3 gamma, corresponding to a region within the internal amino acids.
Purification:The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:Unconjugated
MW:28 KD
Shipping Conditions:Ship on cold packs
Storage:Store at -20 C. Stable for 12 months from date of receipt.
Usage:Research use only

 

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DF3493 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

DF3493 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

Western blot analysis of extracts from 293, rat brain, mouse lung, rat spleen, using 14-3-3 ? Antibody.

Western blot analysis of extracts from 293, rat brain, mouse lung, rat spleen, using 14-3-3 ? Antibody.

DF3493 staining Hela cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

DF3493 staining Hela cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.