HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates [LY400290] (100 ug) and [LC400290] (20 ug) can be purchased separately from OriGene.