HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYC (Cat# RC201611, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC(Cat# TA500002). Positive lysates [LY400876] (100 ug) and [LC400876] (20 ug) can be purchased separately from OriGene.