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Ordering Details

Cat Number:ATGP2140-500ug
Size:500ug
Concentration:0.25 mg/ml (determined by BCA assay)
Price:£577.00
Quantity:
Shipping:£10.00

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Data Sheet

 

Streptavidin [37-159 a.a.], Recombinant Protein

Partner: ATGen

Applications:SDS-PAGE
Species Reactivity:Streptomyces
MW:15.6kDa (148aa), confirmed by MALDI-TOF
Purity:> 90% by SDS - PAGE
Description:Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Further Information:Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP
Synonym:Streptavidin, STAV
Ascension Number:P22629
NCBI EntrezGene:CAA27265
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.