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Cat Number:AST0904-100ul
Size:100ul
Concentration:1mg/ml
Price:£197.00
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Shipping:£10.00

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Data Sheet
MSDS

 

Streptavidin [1C2], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB (Recombinant protein)
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant Streptavidin (25-183aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~3,000. Recommended starting dilution is 1:2,000.
Synonym:Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.
Ascension Number:P22629
NCBI EntrezGene:CAA27265
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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Recombinant Streptavidin protein (17kDa) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

Recombinant Streptavidin protein (17kDa) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system