Ordering Details
Cat Number:	ASC0632-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

alpha/beta-Synuclein [3B6], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human a-synuclein (119-140aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	alpha-synuclein (amino acids 119-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:	a, b - synuclein, Alpha synuclein, Beta synuclein, NACP, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, PARK1, PARK4, Parkinson disease familial 1, PD1, SNCA, SNCB, SNCB protein, Synuclein alpha, Synuclein beta,
Ascension Number:	P37840, Q16143
NCBI EntrezGene:	NP_000336, NP_003076
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human alpha, beta-Synuclein antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of alpha, beta-Synuclein in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human alpha, beta-Synuclein antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of alpha, beta-Synuclein in C6 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human alpha, beta-Synuclein antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).