AGRP Antibody staining of PFA-perfused cryosection of Human Hypothalamus at 0.05ug/ml. Antigen retrieval with citrate buffer pH 6 at 80°C for 30min, HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.