All lanes : Anti-ACBD4 Antibody (Center) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: rat brain lysateLane 4: T47D whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.