All lanes : Anti-ALDH1L2 Antibody (C-term) at 1:2000 dilutionLane 1: Mouse pancreas whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Rat liver whole lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.