Detection of Endogenous STAT6: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblots was probed with mab STAT6-8C12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431. Lane 2: SW480 Lane 3: SW620 Lane 4: HT29. Lane 5: MCF7. Lane 6: MDA-MB231 Lane 7: T47D