HSP27 (HSPB1) pSer82 (incl. pos. control) [5B9], Mouse Monoclonal Antibody

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HSP 27 activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab hsp27-5B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF