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Cat Number:AM00045PU-N
Size:100ug
Price:£468.00
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Shipping:£12.00

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Data Sheet
MSDS

 

EGFR (N-term) (incl. pos. control) [14C8], Mouse Monoclonal Antibody

Partner: OriGene Technologies

Applications:WB
Species Reactivity:Canine, Human, Mouse
Isotype:Mouse IgG1
Description:EGFR (N-term) (incl. pos. control) mouse monoclonal antibody, clone 14C8, Purified
Ascension Number:NP_005219.2
Shipping Conditions:Ship on cold packs
Usage:Research Use Only

 

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Detection of endogenous EGFR: Whole cell lysates of EGF stim ulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-14C8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2

Detection of endogenous EGFR: Whole cell lysates of EGF stim ulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-14C8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2