EGFR Transactivation: Serum starved HepG2 cells were treated for 15min as indicated. Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-1H9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: PMA Lane 3: Forskolin Lane 4: LPA Lane 5: Sorbit Lane 6; Anisomycin Lane 7: Ionomycin Lane 8: Taxol