Antibody sensitivity Whole cell lysates of vanadate - treated HepG2 containing defined cell numbers per lane were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab EGFR-16F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells