Whole cell extracts of control (1) or pervanadate treated (2)OVCAR 5 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with antibody AM00016PU-N (clone 1B11) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).