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Cat Number:AF6431-100ul
Size:100uL
Concentration:1mg/mL
Price:£215.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

4E-BP1, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:WB, IHC, IF/ICC, ELISA
Species Reactivity:Human, Mouse, Rat
Isotype:Rabbit IgG
Immunogen:A synthesized peptide derived from human 4E-BP1, corresponding to a region within the internal amino acids.
Purification:The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:Unconjugated
MW:18kDa
Shipping Conditions:Ship on cold packs
Storage:Store at -20 C. Stable for 12 months from date of receipt.
Usage:Research use only

 

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AF6431 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

AF6431 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

AF6431 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

AF6431 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

Western blot analysis of extracts from Myeloma cells, using 4E-BP1 Antibody. Lane 1 was treated with the blocking peptide.

Western blot analysis of extracts from Myeloma cells, using 4E-BP1 Antibody. Lane 1 was treated with the blocking peptide.