AF6356 at 1/100 staining human liver tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at136��
Western blot analysis of extracts from various samples, using 14-3-3 zeta Antibody. Lane 1: 293 treated with blocking peptide; Lane 2: 293; Lane 3: B16F10.
AF6356 staining HepG2? cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
