AF6276 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 57��
Western blot analysis of extracts from various samples, using ADD1 Antibody. Lane 1: Rat heart treated with blocking peptide; Lane 2: Rat heart; Lane 3: VERO.
AF6276 staining A-431 cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.