Western blot analysis of ATF2 expression in MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
AF6177 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.