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Cat Number:AF6176-200ul
Size:200uL
Concentration:1mg/mL
Price:£269.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

ATF2, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:Human, Mouse, Rat
Isotype:Rabbit IgG
Immunogen:A synthesized peptide derived from human ATF2, corresponding to a region within N-terminal amino acids.
Purification:The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:Unconjugated
MW:52kDa
Shipping Conditions:Ship on cold packs
Storage:Store at -20 C. Stable for 12 months from date of receipt.
Usage:Research use only

 

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Western blot analysis of ATF2 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Western blot analysis of ATF2 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

AF6176 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

AF6176 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22C.

AF6176 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

AF6176 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.