AF1032 at 1/100 staining mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis on rat liver using alpha-SMA antibody ,The lane on the right is blocked with the antigen-specific peptide.
AF1032 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.