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Cat Number:AF1032-100ul
Size:100uL
Concentration:1mg/mL
Price:£215.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

alpha-SMA, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:Human, Mouse, Rat, Bovine
Isotype:Rabbit IgG
Immunogen:A synthesized peptide derived from human alpha-SMA, corresponding to a region within N-terminal amino acids.
Purification:The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:Unconjugated
MW:45 KD
Shipping Conditions:Ship on cold packs
Storage:Store at -20 C. Stable for 12 months from date of receipt.
Usage:Research use only

 

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AF1032 at 1/100 staining mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

AF1032 at 1/100 staining mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

Western blot analysis on rat liver using alpha-SMA antibody ,The lane on the right is blocked with the antigen-specific peptide.

Western blot analysis on rat liver using alpha-SMA antibody ,The lane on the right is blocked with the antigen-specific peptide.

AF1032 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.

AF1032 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.