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Cat Number:ATGA0409-50ul
Size:50ul
Concentration:1 mg/ml
Price:£118.00
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Shipping:£10.00

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Data Sheet
MSDS

 

Isocitrate Dehydrogenase 1/IDH1 [AT25H10], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human IDH1 (1-414aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD
Ascension Number:O75874
NCBI EntrezGene:NP_005887
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDH1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDH1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of IDH1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IDH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of IDH1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IDH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).