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Cat Number:ATGA0349-50ul
Size:50ul
Concentration:1 mg/ml
Price:£118.00
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Shipping:£10.00

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Data Sheet
MSDS

 

PMVK [AT21C10], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human PMVK (1-192aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase
Ascension Number:Q15126
NCBI EntrezGene:NP_006547
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human  PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).