Your Basket 0

 

Ordering Details

Cat Number:ATGA0348-100ul
Size:100ul
Concentration:1 mg/ml
Price:£197.00
Quantity:
Shipping:£10.00

Document Links

Data Sheet
MSDS

 

GRP94/gp96/HSP90B1 [AT94B9], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human GRP94 (22-803aa) purified from E.coli.
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:94 kDa glucose-regulated protein, 94 kDa glucose-regulated protein, HSP90B1, GP96, TRA1, Tumor rejection antigen 1, GRP94, 94 kDa glucose-regulated protein 94 kDa glucose regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 9, Heat shock protein 90 kDa beta member 1, Stress inducible tumor rejection antigen GP96, Tumor rejection antigen gp96.
Ascension Number:P14625
NCBI EntrezGene:NP_003290
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

Insight Biotechnology Ltd reserves the right to change pricing without notice

 

The Recombinant Human GRP94 and Cell lysates were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human GRP94 antibody.Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.

The Recombinant Human GRP94 and Cell lysates were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human GRP94 antibody.Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.

ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).