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Cat Number:ATGA0345-50ul
Size:50ul
Concentration:1 mg/ml
Price:£118.00
Quantity:
Shipping:£10.00

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Data Sheet
MSDS

 

NPM1 [AT23F1], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human NPM1(1-294aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-stranded nucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions. It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. The protein is phosphorylated. Chromosomal aberrations involving NPM1 were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. It has been found in the cytoplasm in patients with primary acute myelogenous leukemia.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended dilution range for ICC/IF and Flow cytometry is 1:200
Synonym:Nucleophosmin, B23, NPM
Ascension Number:P06748
NCBI EntrezGene:NP_002511
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Human NPM1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM1 antibody (1:1000).

The Recombinant Human NPM1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM1 antibody (1:1000).

ICC/IF analysis of NPM1 in WiDr cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM1 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of NPM1 in WiDr cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM1 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).