Your Basket 0

 

Ordering Details

Cat Number:ATGA0343-50ul
Size:50ul
Concentration:1 mg/ml
Price:£118.00
Quantity:
Shipping:£10.00

Document Links

Data Sheet
MSDS

 

beta-III Tubulin [AT2F5], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human TUBB3 (1-450aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Tubulin Beta 3 is 450 amino acid protein. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Its expression is primarily restricted to central and peripheral nervous system.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytmetry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended dilution range for ICC/IF and Flow cytometry is 1:200.
Synonym:Tubulin beta 3 class III, Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TuBB4
Ascension Number:Q13509
NCBI EntrezGene:NP_006077
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

Insight Biotechnology Ltd reserves the right to change pricing without notice

 

The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000).

The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000).

ICC/IF analysis of Tubulin Beta 3 in U-87MG cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Tubulin Beta 3 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of Tubulin Beta 3 in U-87MG cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Tubulin Beta 3 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).