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Cat Number:ATGA0341-100ul
Size:100ul
Concentration:1 mg/ml
Price:£197.00
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Shipping:£10.00

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Data Sheet
MSDS

 

ALDOA [AT3F9], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human ALDOA (1-364aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney, and intestine and similar to aldolase C levels in brain and other nervous tissue.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended dilution range for ICC/IF and Flow cytometry is 1:200.
Synonym:Fructose bisphosphate aldolase A, ALDOA, ALDA, GSD12, Fructose bisphosphate aldolase A Aldolase A, Aldolase A fructose bisphosphatase, Aldolase A fructose bisphosphate, Fructose 1 6 bisphosphate triosephosphate lyase, Fructose bisphosphate aldolase, Lung cancer antigen NY Lu 1, MGC10942, MGC17716, MGC17767, Muscle type aldolase,
Ascension Number:P04075
NCBI EntrezGene:NP_908930
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an EC

The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an EC

ICC/IF analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).