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Cat Number:ATGA0339-100ul
Size:100ul
Concentration:1 mg/ml
Price:£197.00
Quantity:
Shipping:£10.00

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Data Sheet
MSDS

 

CD14 [AT87H7], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, FACS
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human CD14 (20-349aa) purified from E.coli.
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48kDa) or is directly secreted from intracellular vesicles (56kDa). The x-ray crystal structure of human CD14 reveals a monomeric, bent solenoid structure containing a hydrophobic amino-terminal pocket. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid.
Further Information:The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 and ICC/IF, Flow cytometry is 1:100.
Synonym:monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Ascension Number:P08571
NCBI EntrezGene:NP_001167576
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.