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Cat Number:ATGA0337-100ul
Size:100ul
Concentration:1 mg/ml
Price:£197.00
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Shipping:£10.00

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Data Sheet
MSDS

 

KRT20 [AT10D8], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human KRT20 (1-424aa) purified from E.coli.
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:3000, ICC/IF and Flow cytometry is 1:200
Synonym:Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20; CK-20; CK20; K20; KRT21
Ascension Number:P35900
NCBI EntrezGene:NP_061883
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000).

The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000).

ICC/IF analysis of KRT20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of KRT20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).