Your Basket 0

 

Ordering Details

Cat Number:ATGA0260-50ul
Size:50ul
Concentration:1mg/ml
Price:£118.00
Quantity:
Shipping:£10.00

Document Links

Data Sheet
MSDS

 

CTLA-4 [AT4C8], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human CTLA4 (36-161aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.
Further Information:The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, CD, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
Ascension Number:P16410
NCBI EntrezGene:NP_005205
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

Insight Biotechnology Ltd reserves the right to change pricing without notice

 

The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:1000).

The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:1000).

ICC/IF analysis of CTLA4 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTLA4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of CTLA4 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTLA4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).