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Cat Number:ATGA0121-100ul
Size:100ul
Concentration:1mg/ml
Price:£197.00
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Shipping:£10.00

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Data Sheet
MSDS

 

KCTD15 [AT2B11], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human KCTD15(1-159aa) purified from E. coli.
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628
Ascension Number:Q96SI1
NCBI EntrezGene:NP_076981
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of KCTD15 in HeLa cells. The cell was stained with ATGA0121 (1:100). The secondary antibody (green) was used Alexa Fluor 488.

ICC/IF analysis of KCTD15 in HeLa cells. The cell was stained with ATGA0121 (1:100). The secondary antibody (green) was used Alexa Fluor 488.